von Julia Beckhaus ; Svenja Boekhoff ; Katrin Scheinemann ; Freimut H. Schilling ; Gudrun Fleischhack ; Gerhard Binder ; Brigitte Bison ; Torsten Pietsch ; Carsten Friedrich ; Hermann L. Müller
von Julia Beckhaus ; Carsten Friedrich ; Svenja Boekhoff ; Gabriele Calaminus ; Brigitte Bison ; Maria Eveslage ; Beate Timmermann ; Jörg Flitsch ; Hermann L. Müller
von Dominik Sturm ; David Capper ; Felipe Andreiuolo ; Marco Gessi ; Christian Kölsche ; Annekathrin Reinhardt ; Philipp Sievers ; Annika K. Wefers ; Azadeh Ebrahimi ; Abigail Kora Suwala ; Gerrit H. Gielen ; Martin Sill ; Daniel Schrimpf ; Damian Stichel ; Volker Hovestadt ; Bjarne Daenekas ; Agata Rode ; Stefan Hamelmann ; A. Christopher Previti ; Natalie Jäger ; Ivo Buchhalter ; Mirjam Blattner-Johnson ; Barbara Christine Jones ; Monika Warmuth-Metz ; Brigitte Bison ; Kerstin Grund ; Christian Sutter ; Steffen Hirsch ; Nicola Dikow ; Olaf Witt ; Andreas von Deimling ; Hermann L. Müller ; Torsten Pietsch ; Felix Sahm ; Stefan Pfister ; David T. W. Jones
Cancer epidemiology; Cancer epigenetics; CNS cancer; Diagnostic markers; Paediatric cancer
The large diversity of central nervous system (CNS) tumor types in children and adolescents results in disparate patient outcomes and renders accurate diagnosis challenging. In this study, we prospectively integrated DNA methylation profiling and targeted gene panel sequencing with blinded neuropathological reference diagnostics for a population-based cohort of more than 1,200 newly diagnosed pediatric patients with CNS tumors, to assess their utility in routine neuropathology. We show that the multi-omic integration increased diagnostic accuracy in a substantial proportion of patients through annotation to a refining DNA methylation class (50%), detection of diagnostic or therapeutically relevant genetic alterations (47%) or identification of cancer predisposition syndromes (10%). Discrepant results by neuropathological WHO-based and DNA methylation-based classification (30%) were enriched in histological high-grade gliomas, implicating relevance for current clinical patient management in 5% of all patients. Follow-up (median 2.5 years) suggests improved survival for patients with histological high-grade gliomas displaying lower-grade molecular profiles. These results provide preliminary evidence of the utility of integrating multi-omics in neuropathology for pediatric neuro-oncology.
Nature medicine [New York, NY] : Springer Nature, 1995 29(2023), 4, Seite 917-926 Online-Ressource
von Anna-Maria Neßlauer ; Anne Gläser ; Markus Gräler ; Robby Engelmann ; Brigitte Müller-Hilke ; Marcus Frank ; Christine Burstein ; Arndt Rolfs ; John Neidhardt ; Andreas Wree ; Martin Witt ; Anja U. Bräuer
